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General Information
Title Inacc No : InaCC B36, Scientific Name: Bacillus sp.
Organism Bacillus sp.
Sample Type Microbe
Description
External Links
Description URL
None

Sample Information
Sample Attribute
Sample Name InaCC B36
Sample Title Inacc No : InaCC B36, Scientific Name: Bacillus sp.
Description
Taxonomy ID 1409
Bioproject Id
Locus Tag Prefix
Strain Bacillus sp. PLeW3A
Isolate
Isolation Source Leaf
Host
Lab Host
Biomaterial Provider
Sample Type cell culture
Collection Date N/A
Geographic Location Name Indonesia:South East Sulawesi, Protected Forest Papalia, South Konawe
Latitude Longitude
Environmental Biome
Depth
Altitude
Collected By Agustinus Joko N
Culture Collection Indonesian Culture Collection (InaCC) B36
Genotype
Host Tissue Sampled
Identified By
Mating Type
Passage History
Sample Size
Serotype
Serovar
Specimen Voucher
Temperature 30
Organism 3088
Growth Media NA/TSA
Center Direktorat Pengelolaan Laboratorium, Fasilitas Riset, dan Kawasan Sains dan Teknologi
Lab Pusat Sekuensing
Submitter Lab Pusat Sekuensing - BRIN
Submitted at 26-09-2024
Published at 02-10-2024

Other Information

  • Cell morphology:
    • @ref: 116022
    • Gram stain: positive
    • Cell shape: rod-shaped
    • Motility: no

  • Culture medium:
    • 0:
      • @ref: 41589
      • Name: MEDIUM 313 - for Alicyclobacillus acidoterrestris
      • Growth: yes
      • Composition: Distilled water make up to (500.000 ml);Magnesium sulphate heptahydrate (0.180 g);Calcium chloride dihydrate (0.660 g);Glucose (5.000 g);Yeast extract (1.000 g);Ammonium sulphate (0.200 g);Potassium di-hydrogen phosphate (3.000 g);Agar solution - M0614(50
    • 1:
      • @ref: 1510
      • Name: ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402)
      • Growth: yes
      • Link: https://mediadive.dsmz.de/medium/402
      • Composition: Name: ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402) Composition: Agar 15.0 g/l Glucose 5.0 g/l KH2PO4 3.0 g/l Yeast extract 2.0 g/l MgSO4 x 7 H2O 0.5 g/l CaCl2 x 2 H2O 0.25 g/l (NH4)2SO4 0.2 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water
    • 2:
      • @ref: 116022
      • Name: CIP Medium 313
      • Growth: yes
      • Link: https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=313
  • Culture temp:
    • 0:
      • @ref: 1510
      • Growth: positive
      • Type: growth
      • Temperature: 45
    • 1:
      • @ref: 41589
      • Growth: positive
      • Type: growth
      • Temperature: 50
    • 2:
      • @ref: 116022
      • Growth: positive
      • Type: growth
      • Temperature: 25-55
    • 3:
      • @ref: 116022
      • Growth: no
      • Type: growth
      • Temperature: 10
  • Culture pH:
    • @ref: 116022
    • Ability: no
    • Type: growth
    • PH: 6

  • Metabolite utilization:
    • 0:
      • @ref: 116022
      • Chebi-ID: 4853
      • Metabolite: esculin
      • Utilization activity: -
      • Kind of utilization tested: hydrolysis
    • 1:
      • @ref: 116022
      • Chebi-ID: 606565
      • Metabolite: hippurate
      • Utilization activity: -
      • Kind of utilization tested: hydrolysis
    • 2:
      • @ref: 116022
      • Chebi-ID: 17632
      • Metabolite: nitrate
      • Utilization activity: -
      • Kind of utilization tested: reduction
    • 3:
      • @ref: 116022
      • Chebi-ID: 16301
      • Metabolite: nitrite
      • Utilization activity: -
      • Kind of utilization tested: reduction
  • Metabolite production:
    • @ref: 116022
    • Chebi-ID: 35581
    • Metabolite: indole
    • Production: no
  • Metabolite tests:
    • 0:
      • @ref: 116022
      • Chebi-ID: 15688
      • Metabolite: acetoin
      • Voges-proskauer-test: -
    • 1:
      • @ref: 116022
      • Chebi-ID: 17234
      • Metabolite: glucose
      • Methylred-test: -
  • Enzymes:
    • 0:
      • @ref: 68382
      • Value: alpha-mannosidase
      • Activity: -
      • Ec: 3.2.1.24
    • 1:
      • @ref: 68382
      • Value: beta-glucosidase
      • Activity: -
      • Ec: 3.2.1.21
    • 2:
      • @ref: 68382
      • Value: N-acetyl-beta-glucosaminidase
      • Activity: -
      • Ec: 3.2.1.52
    • 3:
      • @ref: 68382
      • Value: beta-galactosidase
      • Activity: -
      • Ec: 3.2.1.23
    • 4:
      • @ref: 68382
      • Value: beta-glucuronidase
      • Activity: -
      • Ec: 3.2.1.31
    • 5:
      • @ref: 68382
      • Value: acid phosphatase
      • Activity: +
      • Ec: 3.1.3.2
    • 6:
      • @ref: 68382
      • Value: naphthol-AS-BI-phosphohydrolase
      • Activity: +
    • 7:
      • @ref: 68382
      • Value: alpha-chymotrypsin
      • Activity: -
      • Ec: 3.4.21.1
    • 8:
      • @ref: 68382
      • Value: trypsin
      • Activity: +
      • Ec: 3.4.21.4
    • 9:
      • @ref: 68382
      • Value: valine arylamidase
      • Activity: +
    • 10:
      • @ref: 68382
      • Value: cystine arylamidase
      • Activity: -
      • Ec: 3.4.11.3
    • 11:
      • @ref: 68382
      • Value: leucine arylamidase
      • Activity: +
      • Ec: 3.4.11.1
    • 12:
      • @ref: 68382
      • Value: lipase (C 14)
      • Activity: -
    • 13:
      • @ref: 68382
      • Value: esterase (C 4)
      • Activity: +
    • 14:
      • @ref: 68382
      • Value: esterase lipase (C 8)
      • Activity: +
    • 15:
      • @ref: 116022
      • Value: oxidase
      • Activity: -
    • 16:
      • @ref: 116022
      • Value: beta-galactosidase
      • Activity: -
      • Ec: 3.2.1.23
    • 17:
      • @ref: 116022
      • Value: alcohol dehydrogenase
      • Activity: -
      • Ec: 1.1.1.1
    • 18:
      • @ref: 116022
      • Value: catalase
      • Activity: +
      • Ec: 1.11.1.6
    • 19:
      • @ref: 116022
      • Value: gamma-glutamyltransferase
      • Activity: -
      • Ec: 2.3.2.2
    • 20:
      • @ref: 116022
      • Value: lysine decarboxylase
      • Activity: -
      • Ec: 4.1.1.18
    • 21:
      • @ref: 116022
      • Value: ornithine decarboxylase
      • Activity: -
      • Ec: 4.1.1.17
    • 22:
      • @ref: 116022
      • Value: urease
      • Activity: -
      • Ec: 3.5.1.5
    • 23:
      • @ref: 68382
      • Value: alpha-fucosidase
      • Activity: -
      • Ec: 3.2.1.51
    • 24:
      • @ref: 68382
      • Value: alpha-glucosidase
      • Activity: -
      • Ec: 3.2.1.20
    • 25:
      • @ref: 68382
      • Value: alpha-galactosidase
      • Activity: -
      • Ec: 3.2.1.22
    • 26:
      • @ref: 68382
      • Value: alkaline phosphatase
      • Activity: -
      • Ec: 3.1.3.1
  • API zym:
    • @ref: 116022
    • Control: -
    • Alkaline phosphatase: -
    • Esterase: +
    • Esterase Lipase: +
    • Lipase: -
    • Leucine arylamidase: +
    • Valine arylamidase: +
    • Cystine arylamidase: -
    • Trypsin: +
    • Alpha- Chymotrypsin: -
    • Acid phosphatase: +
    • Naphthol-AS-BI-phosphohydrolase: +
    • Alpha- Galactosidase: -
    • Beta- Galactosidase: -
    • Beta- Glucuronidase: -
    • Alpha- Glucosidase: -
    • Beta- Glucosidase: -
    • N-acetyl-beta- glucosaminidase: -
    • Alpha- Mannosidase: -
    • Alpha- Fucosidase: -
  • API biotype100:
    • @ref: 116022
    • GLU: -
    • FRU: -
    • GAL: -
    • TRE: -
    • MNE: -
    • SBE: -
    • MEL: -
    • SAC: -
    • RAF: -
    • MTE: -
    • MAL: -
    • LAC: -
    • LTE: -
    • MbGa: -
    • MaGa: -
    • CEL: -
    • GEN: -
    • MbGu: -
    • ESC: -
    • RIB: -
    • ARA: -
    • XYL: -
    • PLE: -
    • RHA: -
    • FUC: -
    • MLZ: -
    • DARL: -
    • LARL: -
    • XLT: -
    • DUL: -
    • TAG: -
    • GLY: -
    • INO: -
    • MAN: -
    • MTL: -
    • TUR: -
    • SOR: -
    • ADO: -
    • HBG: -
    • LYX: -
    • ERY: -
    • MDG: -
    • 3MDG: -
    • SAT: -
    • MUC: -
    • LTAT: -
    • DTAT: -
    • MTAT: -
    • DMLT: -
    • LMLT: -
    • CATE: -
    • TATE: -
    • TTE: -
    • CIT: -
    • GRT: -
    • GAT: -
    • 2KG: -
    • 5KG: -
    • TRY: -
    • NAG: -
    • GNT: -
    • PAC: -
    • PAT: -
    • POBE: -
    • QAT: -
    • GTE: -
    • MOBE: -
    • BAT: -
    • PPAT: -
    • CMT: -
    • TGE: -
    • BET: -
    • PCE: -
    • ABT: -
    • HIN: -
    • LAT: -
    • CAP: -
    • CYT: -
    • HIS: -
    • SUC: -
    • FUM: -
    • GRE: -
    • GYT: -
    • AVT: -
    • ETN: -
    • TTN: -
    • GLN: -
    • ITA: -
    • 3OBU: -
    • APT: -
    • GTT: -
    • PRO: -
    • DALA: -
    • LALA: -
    • SER: -
    • MNT: -
    • PROP: -
    • TYR: -
    • 2KT: -

  • Isolation:
    • 0:
      • @ref: 1510
      • Sample type: garden soil
    • 1:
      • @ref: 116022
      • Sample type: Environment, Garden soil
      • Country: Germany
      • Origin.country: DEU
      • Continent: Europe
  • Isolation source categories:
    • 0:
      • Cat1: #Environmental
      • Cat2: #Terrestrial
      • Cat3: #Soil
    • 1:
      • Cat1: #Engineered
      • Cat2: #Agriculture
      • Cat3: #Garden

  • Risk assessment:
    • 0:
      • @ref: 1510
      • Biosafety level: 1
      • Biosafety level comment: Risk group (German classification)
    • 1:
      • @ref: 116022
      • Biosafety level: 1
      • Biosafety level comment: Risk group (French classification)

  • 16S sequences:
    • 0:
      • @ref: 20218
      • Description: Alicyclobacillus acidoterrestris gene for 16S ribosomal RNA, partial sequence
      • Accession: AB042057
      • Length: 1514
      • Database: nuccore
      • NCBI tax ID: 1450
    • 1:
      • @ref: 20218
      • Description: Alicyclobacillus acidoterrestris strain ATCC 49025 16S ribosomal RNA gene, partial sequence
      • Accession: AY573797
      • Length: 1494
      • Database: nuccore
      • NCBI tax ID: 1450
    • 2:
      • @ref: 20218
      • Description: Alicyclobacillus acidoterrestris 16S rRNA gene, strain DSM 3922T
      • Accession: AJ133631
      • Length: 1482
      • Database: nuccore
      • NCBI tax ID: 1450
    • 3:
      • @ref: 20218
      • Description: Alicyclobacillus acidoterrestris isolate Aaci36 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence
      • Accession: EU723608
      • Length: 564
      • Database: nuccore
      • NCBI tax ID: 1450
    • 4:
      • @ref: 20218
      • Description: Alicyclobacillus acidoterrestris isolate Aaci39 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence
      • Accession: EU723609
      • Length: 441
      • Database: nuccore
      • NCBI tax ID: 1450
    • 5:
      • @ref: 20218
      • Description: Alicyclobacillus acidoterrestris strain DSM 3922 16S ribosomal RNA gene, partial sequence
      • Accession: KF880724
      • Length: 1421
      • Database: nuccore
      • NCBI tax ID: 1450
    • 6:
      • @ref: 20218
      • Description: Bacillus sp. 16S ribosomal RNA
      • Accession: X60602
      • Length: 1432
      • Database: nuccore
      • NCBI tax ID: 1409
  • Genome sequences:
    • 0:
      • @ref: 66792
      • Description: Alicyclobacillus acidoterrestris ATCC 49025
      • Accession: GCA_000444055
      • Assembly level: contig
      • Database: ncbi
      • NCBI tax ID: 1356854
    • 1:
      • @ref: 66792
      • Description: Alicyclobacillus acidoterrestris ATCC 49025
      • Accession: 1356854.4
      • Assembly level: wgs
      • Database: patric
      • NCBI tax ID: 1356854
    • 2:
      • @ref: 66792
      • Description: Alicyclobacillus acidoterrestris ATCC 49025
      • Accession: 2558860250
      • Assembly level: draft
      • Database: img
      • NCBI tax ID: 1356854
    • 3:
      • @ref: 66792
      • Description: Alicyclobacillus acidoterrestris DSM 3922
      • Accession: GCA_022674245
      • Assembly level: complete
      • Database: ncbi
      • NCBI tax ID: 1450
  • GC content:
    • @ref: 1510
    • GC-content: 51.6
    • Method: thermal denaturation, midpoint method (Tm)

  • Lineage: cellular organisms; Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: firmicutes)
  • LineageEx:
    • Taxon:
      • 0:
        • TaxId: 131567
        • ScientificName: cellular organisms
        • Rank: cellular root
      • 1:
        • TaxId: 2
        • ScientificName: Bacteria
        • Rank: domain
      • 2:
        • TaxId: 1783272
        • ScientificName: Bacillati
        • Rank: kingdom
      • 3:
        • TaxId: 1239
        • ScientificName: Bacillota
        • Rank: phylum
      • 4:
        • TaxId: 91061
        • ScientificName: Bacilli
        • Rank: class
      • 5:
        • TaxId: 1385
        • ScientificName: Bacillales
        • Rank: order
      • 6:
        • TaxId: 186817
        • ScientificName: Bacillaceae
        • Rank: family
      • 7:
        • TaxId: 1386
        • ScientificName: Bacillus
        • Rank: genus
      • 8:
        • TaxId: 185979
        • ScientificName: unclassified Bacillus (in: firmicutes)
        • Rank: no rank