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General Information
Title Inacc No : InaCC B1548, Scientific Name: Bacillus sp.
Organism Bacillus sp.
Sample Type Microbe
Description
External Links
Description URL
None

Sample Information
Sample Attribute
Sample Name InaCC B1548
Sample Title Inacc No : InaCC B1548, Scientific Name: Bacillus sp.
Description
Taxonomy ID 1409
Bioproject Id
Locus Tag Prefix
Strain Bacillus sp. D3.3
Isolate
Isolation Source Straw mushroom cultivation medium
Host
Lab Host
Biomaterial Provider
Sample Type cell culture
Collection Date N/A
Geographic Location Name Indonesia: West Java, Jatisari, Karawang
Latitude Longitude
Environmental Biome
Depth
Altitude
Collected By Masrukhin
Culture Collection Indonesian Culture Collection (InaCC) B1548
Genotype
Host Tissue Sampled
Identified By
Mating Type
Passage History
Sample Size
Serotype
Serovar
Specimen Voucher
Temperature 25-30
Organism 3088
Growth Media NA
Center Direktorat Pengelolaan Laboratorium, Fasilitas Riset, dan Kawasan Sains dan Teknologi
Lab Pusat Sekuensing
Submitter Lab Pusat Sekuensing - BRIN
Submitted at 26-09-2024
Published at 02-10-2024

Other Information

  • Cell morphology:
    • @ref: 116022
    • Gram stain: positive
    • Cell shape: rod-shaped
    • Motility: no

  • Culture medium:
    • 0:
      • @ref: 41589
      • Name: MEDIUM 313 - for Alicyclobacillus acidoterrestris
      • Growth: yes
      • Composition: Distilled water make up to (500.000 ml);Magnesium sulphate heptahydrate (0.180 g);Calcium chloride dihydrate (0.660 g);Glucose (5.000 g);Yeast extract (1.000 g);Ammonium sulphate (0.200 g);Potassium di-hydrogen phosphate (3.000 g);Agar solution - M0614(50
    • 1:
      • @ref: 1510
      • Name: ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402)
      • Growth: yes
      • Link: https://mediadive.dsmz.de/medium/402
      • Composition: Name: ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402) Composition: Agar 15.0 g/l Glucose 5.0 g/l KH2PO4 3.0 g/l Yeast extract 2.0 g/l MgSO4 x 7 H2O 0.5 g/l CaCl2 x 2 H2O 0.25 g/l (NH4)2SO4 0.2 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water
    • 2:
      • @ref: 116022
      • Name: CIP Medium 313
      • Growth: yes
      • Link: https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=313
  • Culture temp:
    • 0:
      • @ref: 1510
      • Growth: positive
      • Type: growth
      • Temperature: 45
    • 1:
      • @ref: 41589
      • Growth: positive
      • Type: growth
      • Temperature: 50
    • 2:
      • @ref: 116022
      • Growth: positive
      • Type: growth
      • Temperature: 25-55
    • 3:
      • @ref: 116022
      • Growth: no
      • Type: growth
      • Temperature: 10
  • Culture pH:
    • @ref: 116022
    • Ability: no
    • Type: growth
    • PH: 6

  • Metabolite utilization:
    • 0:
      • @ref: 116022
      • Chebi-ID: 4853
      • Metabolite: esculin
      • Utilization activity: -
      • Kind of utilization tested: hydrolysis
    • 1:
      • @ref: 116022
      • Chebi-ID: 606565
      • Metabolite: hippurate
      • Utilization activity: -
      • Kind of utilization tested: hydrolysis
    • 2:
      • @ref: 116022
      • Chebi-ID: 17632
      • Metabolite: nitrate
      • Utilization activity: -
      • Kind of utilization tested: reduction
    • 3:
      • @ref: 116022
      • Chebi-ID: 16301
      • Metabolite: nitrite
      • Utilization activity: -
      • Kind of utilization tested: reduction
  • Metabolite production:
    • @ref: 116022
    • Chebi-ID: 35581
    • Metabolite: indole
    • Production: no
  • Metabolite tests:
    • 0:
      • @ref: 116022
      • Chebi-ID: 15688
      • Metabolite: acetoin
      • Voges-proskauer-test: -
    • 1:
      • @ref: 116022
      • Chebi-ID: 17234
      • Metabolite: glucose
      • Methylred-test: -
  • Enzymes:
    • 0:
      • @ref: 68382
      • Value: alpha-mannosidase
      • Activity: -
      • Ec: 3.2.1.24
    • 1:
      • @ref: 68382
      • Value: beta-glucosidase
      • Activity: -
      • Ec: 3.2.1.21
    • 2:
      • @ref: 68382
      • Value: N-acetyl-beta-glucosaminidase
      • Activity: -
      • Ec: 3.2.1.52
    • 3:
      • @ref: 68382
      • Value: beta-galactosidase
      • Activity: -
      • Ec: 3.2.1.23
    • 4:
      • @ref: 68382
      • Value: beta-glucuronidase
      • Activity: -
      • Ec: 3.2.1.31
    • 5:
      • @ref: 68382
      • Value: acid phosphatase
      • Activity: +
      • Ec: 3.1.3.2
    • 6:
      • @ref: 68382
      • Value: naphthol-AS-BI-phosphohydrolase
      • Activity: +
    • 7:
      • @ref: 68382
      • Value: alpha-chymotrypsin
      • Activity: -
      • Ec: 3.4.21.1
    • 8:
      • @ref: 68382
      • Value: trypsin
      • Activity: +
      • Ec: 3.4.21.4
    • 9:
      • @ref: 68382
      • Value: valine arylamidase
      • Activity: +
    • 10:
      • @ref: 68382
      • Value: cystine arylamidase
      • Activity: -
      • Ec: 3.4.11.3
    • 11:
      • @ref: 68382
      • Value: leucine arylamidase
      • Activity: +
      • Ec: 3.4.11.1
    • 12:
      • @ref: 68382
      • Value: lipase (C 14)
      • Activity: -
    • 13:
      • @ref: 68382
      • Value: esterase (C 4)
      • Activity: +
    • 14:
      • @ref: 68382
      • Value: esterase lipase (C 8)
      • Activity: +
    • 15:
      • @ref: 116022
      • Value: oxidase
      • Activity: -
    • 16:
      • @ref: 116022
      • Value: beta-galactosidase
      • Activity: -
      • Ec: 3.2.1.23
    • 17:
      • @ref: 116022
      • Value: alcohol dehydrogenase
      • Activity: -
      • Ec: 1.1.1.1
    • 18:
      • @ref: 116022
      • Value: catalase
      • Activity: +
      • Ec: 1.11.1.6
    • 19:
      • @ref: 116022
      • Value: gamma-glutamyltransferase
      • Activity: -
      • Ec: 2.3.2.2
    • 20:
      • @ref: 116022
      • Value: lysine decarboxylase
      • Activity: -
      • Ec: 4.1.1.18
    • 21:
      • @ref: 116022
      • Value: ornithine decarboxylase
      • Activity: -
      • Ec: 4.1.1.17
    • 22:
      • @ref: 116022
      • Value: urease
      • Activity: -
      • Ec: 3.5.1.5
    • 23:
      • @ref: 68382
      • Value: alpha-fucosidase
      • Activity: -
      • Ec: 3.2.1.51
    • 24:
      • @ref: 68382
      • Value: alpha-glucosidase
      • Activity: -
      • Ec: 3.2.1.20
    • 25:
      • @ref: 68382
      • Value: alpha-galactosidase
      • Activity: -
      • Ec: 3.2.1.22
    • 26:
      • @ref: 68382
      • Value: alkaline phosphatase
      • Activity: -
      • Ec: 3.1.3.1
  • API zym:
    • @ref: 116022
    • Control: -
    • Alkaline phosphatase: -
    • Esterase: +
    • Esterase Lipase: +
    • Lipase: -
    • Leucine arylamidase: +
    • Valine arylamidase: +
    • Cystine arylamidase: -
    • Trypsin: +
    • Alpha- Chymotrypsin: -
    • Acid phosphatase: +
    • Naphthol-AS-BI-phosphohydrolase: +
    • Alpha- Galactosidase: -
    • Beta- Galactosidase: -
    • Beta- Glucuronidase: -
    • Alpha- Glucosidase: -
    • Beta- Glucosidase: -
    • N-acetyl-beta- glucosaminidase: -
    • Alpha- Mannosidase: -
    • Alpha- Fucosidase: -
  • API biotype100:
    • @ref: 116022
    • GLU: -
    • FRU: -
    • GAL: -
    • TRE: -
    • MNE: -
    • SBE: -
    • MEL: -
    • SAC: -
    • RAF: -
    • MTE: -
    • MAL: -
    • LAC: -
    • LTE: -
    • MbGa: -
    • MaGa: -
    • CEL: -
    • GEN: -
    • MbGu: -
    • ESC: -
    • RIB: -
    • ARA: -
    • XYL: -
    • PLE: -
    • RHA: -
    • FUC: -
    • MLZ: -
    • DARL: -
    • LARL: -
    • XLT: -
    • DUL: -
    • TAG: -
    • GLY: -
    • INO: -
    • MAN: -
    • MTL: -
    • TUR: -
    • SOR: -
    • ADO: -
    • HBG: -
    • LYX: -
    • ERY: -
    • MDG: -
    • 3MDG: -
    • SAT: -
    • MUC: -
    • LTAT: -
    • DTAT: -
    • MTAT: -
    • DMLT: -
    • LMLT: -
    • CATE: -
    • TATE: -
    • TTE: -
    • CIT: -
    • GRT: -
    • GAT: -
    • 2KG: -
    • 5KG: -
    • TRY: -
    • NAG: -
    • GNT: -
    • PAC: -
    • PAT: -
    • POBE: -
    • QAT: -
    • GTE: -
    • MOBE: -
    • BAT: -
    • PPAT: -
    • CMT: -
    • TGE: -
    • BET: -
    • PCE: -
    • ABT: -
    • HIN: -
    • LAT: -
    • CAP: -
    • CYT: -
    • HIS: -
    • SUC: -
    • FUM: -
    • GRE: -
    • GYT: -
    • AVT: -
    • ETN: -
    • TTN: -
    • GLN: -
    • ITA: -
    • 3OBU: -
    • APT: -
    • GTT: -
    • PRO: -
    • DALA: -
    • LALA: -
    • SER: -
    • MNT: -
    • PROP: -
    • TYR: -
    • 2KT: -

  • Isolation:
    • 0:
      • @ref: 1510
      • Sample type: garden soil
    • 1:
      • @ref: 116022
      • Sample type: Environment, Garden soil
      • Country: Germany
      • Origin.country: DEU
      • Continent: Europe
  • Isolation source categories:
    • 0:
      • Cat1: #Environmental
      • Cat2: #Terrestrial
      • Cat3: #Soil
    • 1:
      • Cat1: #Engineered
      • Cat2: #Agriculture
      • Cat3: #Garden

  • Risk assessment:
    • 0:
      • @ref: 1510
      • Biosafety level: 1
      • Biosafety level comment: Risk group (German classification)
    • 1:
      • @ref: 116022
      • Biosafety level: 1
      • Biosafety level comment: Risk group (French classification)

  • 16S sequences:
    • 0:
      • @ref: 20218
      • Description: Alicyclobacillus acidoterrestris gene for 16S ribosomal RNA, partial sequence
      • Accession: AB042057
      • Length: 1514
      • Database: nuccore
      • NCBI tax ID: 1450
    • 1:
      • @ref: 20218
      • Description: Alicyclobacillus acidoterrestris strain ATCC 49025 16S ribosomal RNA gene, partial sequence
      • Accession: AY573797
      • Length: 1494
      • Database: nuccore
      • NCBI tax ID: 1450
    • 2:
      • @ref: 20218
      • Description: Alicyclobacillus acidoterrestris 16S rRNA gene, strain DSM 3922T
      • Accession: AJ133631
      • Length: 1482
      • Database: nuccore
      • NCBI tax ID: 1450
    • 3:
      • @ref: 20218
      • Description: Alicyclobacillus acidoterrestris isolate Aaci36 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence
      • Accession: EU723608
      • Length: 564
      • Database: nuccore
      • NCBI tax ID: 1450
    • 4:
      • @ref: 20218
      • Description: Alicyclobacillus acidoterrestris isolate Aaci39 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence
      • Accession: EU723609
      • Length: 441
      • Database: nuccore
      • NCBI tax ID: 1450
    • 5:
      • @ref: 20218
      • Description: Alicyclobacillus acidoterrestris strain DSM 3922 16S ribosomal RNA gene, partial sequence
      • Accession: KF880724
      • Length: 1421
      • Database: nuccore
      • NCBI tax ID: 1450
    • 6:
      • @ref: 20218
      • Description: Bacillus sp. 16S ribosomal RNA
      • Accession: X60602
      • Length: 1432
      • Database: nuccore
      • NCBI tax ID: 1409
  • Genome sequences:
    • 0:
      • @ref: 66792
      • Description: Alicyclobacillus acidoterrestris ATCC 49025
      • Accession: GCA_000444055
      • Assembly level: contig
      • Database: ncbi
      • NCBI tax ID: 1356854
    • 1:
      • @ref: 66792
      • Description: Alicyclobacillus acidoterrestris ATCC 49025
      • Accession: 1356854.4
      • Assembly level: wgs
      • Database: patric
      • NCBI tax ID: 1356854
    • 2:
      • @ref: 66792
      • Description: Alicyclobacillus acidoterrestris ATCC 49025
      • Accession: 2558860250
      • Assembly level: draft
      • Database: img
      • NCBI tax ID: 1356854
    • 3:
      • @ref: 66792
      • Description: Alicyclobacillus acidoterrestris DSM 3922
      • Accession: GCA_022674245
      • Assembly level: complete
      • Database: ncbi
      • NCBI tax ID: 1450
  • GC content:
    • @ref: 1510
    • GC-content: 51.6
    • Method: thermal denaturation, midpoint method (Tm)

  • Lineage: cellular organisms; Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: firmicutes)
  • LineageEx:
    • Taxon:
      • 0:
        • TaxId: 131567
        • ScientificName: cellular organisms
        • Rank: cellular root
      • 1:
        • TaxId: 2
        • ScientificName: Bacteria
        • Rank: domain
      • 2:
        • TaxId: 1783272
        • ScientificName: Bacillati
        • Rank: kingdom
      • 3:
        • TaxId: 1239
        • ScientificName: Bacillota
        • Rank: phylum
      • 4:
        • TaxId: 91061
        • ScientificName: Bacilli
        • Rank: class
      • 5:
        • TaxId: 1385
        • ScientificName: Bacillales
        • Rank: order
      • 6:
        • TaxId: 186817
        • ScientificName: Bacillaceae
        • Rank: family
      • 7:
        • TaxId: 1386
        • ScientificName: Bacillus
        • Rank: genus
      • 8:
        • TaxId: 185979
        • ScientificName: unclassified Bacillus (in: firmicutes)
        • Rank: no rank