BioRepo | Biosample

Other Information

Key: 4439318286
DatasetKey: 7e9d3bc6-bb85-42a5-8826-fe2b27552e2c
PublishingOrgKey: 10edba3c-8fe0-4a8c-8767-312d0e2badb5
InstallationKey: f66ca88d-1f6a-49dc-b33b-32960faee57e
HostingOrganizationKey: fbca90e3-8aed-48b1-84e3-369afbd000ce
PublishingCountry: LK
Protocol: EML
LastCrawled: 2024-07-02T10:12:33.795+00:00
LastParsed: 2024-07-02T10:14:15.226+00:00
CrawlId: 35
Extensions:
BasisOfRecord: MACHINE_OBSERVATION
IndividualCount: 2
OccurrenceStatus: PRESENT
TaxonKey: 3223612
KingdomKey: 3
PhylumKey: 10813635
ClassKey: 10701801
OrderKey: 10857265
FamilyKey: 8091
GenusKey: 2584528
SpeciesKey: 3223612
AcceptedTaxonKey: 3223612
ScientificName: Streptomyces kunmingensis (Ruan et al., 1985) Goodfellow et al., 1986
AcceptedScientificName: Streptomyces kunmingensis (Ruan et al., 1985) Goodfellow et al., 1986
Kingdom: Bacteria
Phylum: Actinobacteriota
Order: Streptomycetales
Family: Streptomycetaceae
Genus: Streptomyces
Species: Streptomyces kunmingensis
GenericName: Streptomyces
SpecificEpithet: kunmingensis
TaxonRank: SPECIES
TaxonomicStatus: ACCEPTED
IucnRedListCategory: NE
DecimalLatitude: 6.141744
DecimalLongitude: 81.119378
Continent: ASIA
Gadm:
- Level0:
  - Gid: LKA
  - Name: Sri Lanka
- Level1:
  - Gid: LKA.8_1
  - Name: Hambantota
- Level2:
  - Gid: LKA.8.4_1
  - Name: Hambantota
Year: 2022
Month: 5
Day: 29
EventDate: 2022-05-29
StartDayOfYear: 149
EndDayOfYear: 149
Issues:
LastInterpreted: 2024-07-02T10:14:15.226+00:00
License: http://creativecommons.org/publicdomain/zero/1.0/legalcode
OrganismQuantity: 2.0
OrganismQuantityType: individuals
IsSequenced: False
Identifiers:
- 0:
  - Identifier: V4R27_868
Media:
Facts:
Relations:
IsInCluster: False
SamplingProtocol: eDNA sampling from soil
GeodeticDatum: WGS84
Class: Actinomycetia
CountryCode: LK
RecordedByIDs:
IdentifiedByIDs:
Country: Sri Lanka
GbifRegion: ASIA
PublishedByGbifRegion: ASIA
EventID: V4R27
Identifier: V4R27_868
GbifID: 4439318286
OccurrenceID: V4R27_868

Colony morphology:
- 0:
  - @ref: 19522
  - Colony color: Brown
  - Incubation period: 10-14 days
  - Medium used: ISP 2
- 1:
  - @ref: 19522
  - Colony color: Beige
  - Incubation period: 10-14 days
  - Medium used: ISP 3
- 2:
  - @ref: 19522
  - Colony color: Red
  - Incubation period: 10-14 days
  - Medium used: ISP 4
- 3:
  - @ref: 19522
  - Colony color: Brown
  - Incubation period: 10-14 days
  - Medium used: ISP 5
- 4:
  - @ref: 19522
  - Colony color: Beige
  - Incubation period: 10-14 days
  - Medium used: ISP 6
- 5:
  - @ref: 19522
  - Colony color: Brown
  - Incubation period: 10-14 days
  - Medium used: ISP 7
Multicellular morphology:
- 0:
  - @ref: 19522
  - Forms multicellular complex: yes
  - Complex name: Aerial Mycelium
  - Complex color: White
  - Medium name: ISP 2
- 1:
  - @ref: 19522
  - Forms multicellular complex: yes
  - Complex name: Aerial Mycelium
  - Complex color: Brown
  - Medium name: ISP 3
- 2:
  - @ref: 19522
  - Forms multicellular complex: yes
  - Complex name: Aerial Mycelium
  - Complex color: White
  - Medium name: ISP 4
- 3:
  - @ref: 19522
  - Forms multicellular complex: yes
  - Complex name: Aerial Mycelium
  - Complex color: White
  - Medium name: ISP 5
- 4:
  - @ref: 19522
  - Forms multicellular complex: no
  - Medium name: ISP 6
- 5:
  - @ref: 19522
  - Forms multicellular complex: yes
  - Complex name: Aerial Mycelium
  - Complex color: White
  - Medium name: ISP 7

Culture medium:
- 0:
  - @ref: 10464
  - Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)
  - Growth: yes
  - Link: https://mediadive.dsmz.de/medium/65
  - Composition: Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
- 1:
  - @ref: 19522
  - Name: ISP 2
  - Growth: yes
  - Composition: Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
- 2:
  - @ref: 19522
  - Name: ISP 3
  - Growth: yes
  - Composition: Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
- 3:
  - @ref: 19522
  - Name: ISP 4
  - Growth: yes
  - Composition: Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
- 4:
  - @ref: 19522
  - Name: ISP 5
  - Growth: yes
  - Composition: Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
- 5:
  - @ref: 19522
  - Name: ISP 6
  - Growth: yes
  - Composition: Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
- 6:
  - @ref: 19522
  - Name: ISP 7
  - Growth: yes
  - Composition: Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
Culture temp:
- 0:
  - @ref: 10464
  - Growth: positive
  - Type: growth
  - Temperature: 28
- 1:
  - @ref: 19522
  - Growth: positive
  - Type: optimum
  - Temperature: 28
- 2:
  - @ref: 67770
  - Growth: positive
  - Type: growth
  - Temperature: 28

Spore formation:
- @ref: 19522
- Spore description: Formation of spore chains: spirales, spore surface: smooth
- Type of spore: spore
- Spore formation: yes
Halophily:
- @ref: 19522
- Salt: NaCl
- Growth: positive
- Tested relation: maximum
- Concentration: 5 %
Metabolite utilization:
- 0:
  - @ref: 19522
  - Chebi-ID: 62968
  - Metabolite: cellulose
  - Utilization activity: -
- 1:
  - @ref: 19522
  - Chebi-ID: 16634
  - Metabolite: raffinose
  - Utilization activity: -
- 2:
  - @ref: 19522
  - Chebi-ID: 26546
  - Metabolite: rhamnose
  - Utilization activity: +
- 3:
  - @ref: 19522
  - Chebi-ID: 28757
  - Metabolite: fructose
  - Utilization activity: +
- 4:
  - @ref: 19522
  - Chebi-ID: 29864
  - Metabolite: mannitol
  - Utilization activity: +
- 5:
  - @ref: 19522
  - Chebi-ID: 17268
  - Metabolite: myo-inositol
  - Utilization activity: -
- 6:
  - @ref: 19522
  - Chebi-ID: 18222
  - Metabolite: xylose
  - Utilization activity: -
- 7:
  - @ref: 19522
  - Chebi-ID: 17992
  - Metabolite: sucrose
  - Utilization activity: -
- 8:
  - @ref: 19522
  - Chebi-ID: 22599
  - Metabolite: arabinose
  - Utilization activity: +
- 9:
  - @ref: 19522
  - Chebi-ID: 17234
  - Metabolite: glucose
  - Utilization activity: +
- 10:
  - @ref: 68368
  - Chebi-ID: 5291
  - Metabolite: gelatin
  - Utilization activity: +
  - Kind of utilization tested: hydrolysis
- 11:
  - @ref: 68368
  - Chebi-ID: 27897
  - Metabolite: tryptophan
  - Utilization activity: -
  - Kind of utilization tested: energy source
- 12:
  - @ref: 68368
  - Chebi-ID: 16199
  - Metabolite: urea
  - Utilization activity: +
  - Kind of utilization tested: hydrolysis
- 13:
  - @ref: 68368
  - Chebi-ID: 16947
  - Metabolite: citrate
  - Utilization activity: +
  - Kind of utilization tested: assimilation
- 14:
  - @ref: 68368
  - Chebi-ID: 18257
  - Metabolite: ornithine
  - Utilization activity: +
  - Kind of utilization tested: degradation
- 15:
  - @ref: 68368
  - Chebi-ID: 25094
  - Metabolite: lysine
  - Utilization activity: +
  - Kind of utilization tested: degradation
- 16:
  - @ref: 68368
  - Chebi-ID: 29016
  - Metabolite: arginine
  - Utilization activity: +
  - Kind of utilization tested: hydrolysis
Metabolite production:
- 0:
  - @ref: 68368
  - Chebi-ID: 15688
  - Metabolite: acetoin
  - Production: yes
- 1:
  - @ref: 68368
  - Chebi-ID: 35581
  - Metabolite: indole
  - Production: no
- 2:
  - @ref: 68368
  - Chebi-ID: 16136
  - Metabolite: hydrogen sulfide
  - Production: no
Metabolite tests:
- 0:
  - @ref: 68368
  - Chebi-ID: 15688
  - Metabolite: acetoin
  - Voges-proskauer-test: +
- 1:
  - @ref: 68368
  - Chebi-ID: 35581
  - Metabolite: indole
  - Indole test: -
Enzymes:
- 0:
  - @ref: 68368
  - Value: gelatinase
  - Activity: +
- 1:
  - @ref: 68368
  - Value: tryptophan deaminase
  - Activity: -
  - Ec: 4.1.99.1
- 2:
  - @ref: 68368
  - Value: urease
  - Activity: +
  - Ec: 3.5.1.5
- 3:
  - @ref: 68368
  - Value: ornithine decarboxylase
  - Activity: +
  - Ec: 4.1.1.17
- 4:
  - @ref: 68368
  - Value: lysine decarboxylase
  - Activity: +
  - Ec: 4.1.1.18
- 5:
  - @ref: 68368
  - Value: arginine dihydrolase
  - Activity: +
  - Ec: 3.5.3.6
- 6:
  - @ref: 68368
  - Value: beta-galactosidase
  - Activity: +
  - Ec: 3.2.1.23
API 20E:
- @ref: 19522
- ONPG: +
- ADH Arg: +
- LDC Lys: +
- ODC: +
- CIT: +
- H2S: -
- URE: +
- TDA Trp: -
- IND: -
- VP: +
- GEL: +

Isolation:
- 0:
  - @ref: 10464
  - Sample type: soil
  - Geographic location: Kunming, Dagyanlow's Lake
  - Country: China
  - Origin.country: CHN
  - Continent: Asia
- 1:
  - @ref: 67770
  - Sample type: Soil
Isolation source categories:
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil

Risk assessment:
- 0:
  - @ref: 10464
  - Biosafety level: 1
  - Biosafety level comment: Risk group (German classification)
- 1:
  - @ref: 19522
  - Biosafety level: 1
  - Biosafety level comment: risk group (German classification)

16S sequences:
- 0:
  - @ref: 20218
  - Description: Streptomyces kunmingensis gene for 16S ribosomal RNA, partial sequence, strain: JCM 7473
  - Accession: D44441
  - Length: 120
  - Database: nuccore
  - NCBI tax ID: 68225
- 1:
  - @ref: 20218
  - Description: Streptomyces kunmingensis gene for 16S rRNA, partial sequence, strain: NBRC 14463
  - Accession: AB184597
  - Length: 1454
  - Database: nuccore
  - NCBI tax ID: 68225
- 2:
  - @ref: 20218
  - Description: Streptomyces kunmingensis strain NRRL B-16240T 16S ribosomal RNA gene, partial sequence
  - Accession: DQ442513
  - Length: 1487
  - Database: nuccore
  - NCBI tax ID: 68225
GC content:
- @ref: 67770
- GC-content: 71.3
- Method: thermal denaturation, midpoint method (Tm)

Lineage: cellular organisms; Bacteria; Terrabacteria group; Actinomycetota; Actinomycetes; Kitasatosporales; Streptomycetaceae; Streptomyces
LineageEx:
- Taxon:
  - 0:
    - TaxId: 131567
    - ScientificName: cellular organisms
    - Rank: no rank
  - 1:
    - TaxId: 2
    - ScientificName: Bacteria
    - Rank: superkingdom
  - 2:
    - TaxId: 1783272
    - ScientificName: Terrabacteria group
    - Rank: clade
  - 3:
    - TaxId: 201174
    - ScientificName: Actinomycetota
    - Rank: phylum
  - 4:
    - TaxId: 1760
    - ScientificName: Actinomycetes
    - Rank: class
  - 5:
    - TaxId: 85011
    - ScientificName: Kitasatosporales
    - Rank: order
  - 6:
    - TaxId: 2062
    - ScientificName: Streptomycetaceae
    - Rank: family
  - 7:
    - TaxId: 1883
    - ScientificName: Streptomyces
    - Rank: genus

General Information

Sample Information

Other Information

General Information

Sample Information

Other Information

Occurence (geoference records)

Morphology

Culture and growth conditions

Physiology and metabolism

Isolation, sampling and environmental information

Safety information

Sequence information

Name and taxonomic classification